Surveillance for SARS-CoV-2 in Norway Rats (Rattus norvegicus) from Southern Ontario

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from wildlife has raised concerns about spillover from humans to animals, the establishment of novel wildlife reservoirs, and the potential for future outbreaks caused by variants of wildlife origin. Norway rats (Rattus norvegicus) are abundant in urban areas and live in close proximity to humans, providing the opportunity for spillover of SARS-CoV-2. Evidence of SARS-CoV-2 infection and exposure has been reported in Norway rats. We investigated SARS-CoV-2 infection and exposure in Norway rats from Southern Ontario, Canada. From October 2019 to June 2021, 224 rats were submitted by collaborating pest control companies. The majority of samples were collected in Windsor (79.9%;n = 179), Hamilton (13.8%;n = 31), and the Greater Toronto Area (5.8%;n = 13). Overall, 50.0% (n = 112) were female and most rats were sexually mature (55.8%;n = 125). Notably, 202 samples were collected prior to the emergence of variants of concern (VOC) and 22 were collected while the Alpha variant (B.1.1.7) was the predominant circulating VOC in humans. Nasal turbinate (n = 164) and small intestinal (n = 213) tissue samples were analyzed for SARS-CoV-2 RNA by RT-PCR. Thoracic cavity fluid samples (n = 213) were tested for neutralizing antibodies using a surrogate virus neutralization test (sVNT) (GenScript cPass);confirmatory plaque reduction neutralization test (PRNT) was conducted on presumptive positive samples. We did not detect SARS-CoV-2 RNA in any samples tested. Two out of eleven samples positive on sVNT had neutralizing antibodies confirmed positive by PRNT (1 : 40 and 1 : 320 PRNT70);both were collected prior to the emergence of VOC. It is imperative that efforts to control and monitor SARS-CoV-2 include surveillance of rats and other relevant wildlife species as novel variants continue to emerge.

Health risks of pest control and disinfection workers after the COVID-19 outbreak in South Korea

The exposure patterns of pest control and disinfection workers have changed after the coronavirus disease 2019 (COVID-19) outbreak, but the health risks of chemical exposure have not been assessed. We identified these workers' chemical exposure patterns and risks before and after the COVID-19 outbreak. We used data conducted between 2018 (pre-pandemic) and 2021 (post-pandemic) from three-year cross-sectional surveys on pest control and disinfection workers. Inhalation and dermal exposure concentrations were estimated using equations based on a biocidal product risk assessment model from the Korean National Institute of Environmental Research. The non-carcinogenic and carcinogenic risks of chemicals were calculated using the United States Environmental Protection Agency risk assessment model. We found that the annual work frequency (50th percentile) of foggers using disinfectants increased the most among all the work types, from 140 uses/year to 176 uses/year after the COVID-19 outbreak. Moreover, all chemicals' non-carcinogenic and carcinogenic risks increased regardless of exposure routes. In the worst scenario (95th percentile), the margin of exposure for citric acid, benzethonium chloride, benzyl-C12-16-alkyldimethyl chlorides, and sodium chlorite of inhalation exposure, and isopropyl alcohol and benzyl-C12-16-alkyldimethyl chlorides of dermal exposure were acceptable (>100) before the COVID-19 outbreak but became unacceptable (<100) after the COVID-19 outbreak. Carcinogenic risks of dichlorvos from inhalation and dermal exposure were above acceptable levels (>10−6) before and after the COVID-19 outbreak but comparatively high after the COVID-19 outbreak. Additionally, significantly more workers experienced health symptoms after the COVID-19 outbreak (p<0.05), with the most common being muscle lethargy (31%), skin/face stinging (28.7%), and breath shortness/neck pain (24.1%).

Arming the Pacific region against pests and disease

It is reported that networks developed through the ACIAR Pacific Plant Biosecurity Partnership programme continue to help strengthen capacity in the prevention of the spread of pests and disease in crops throughout the Pacific region, despite COVID-19 disrupting the programme. Participants from 9 Pacific countries have developed their skills, networks and capacity to facilitate trade and prevent the spread of disease. Relationships and resources created through the programme continue to provide support for information-sharing around plant pests and diseases.

Protecting the world's plant resources from pests

This Annual Report provides in-depth information, key facts and figures from the global plant health community of the International Plant Protection Convention (IPPC) during the International Year of Plant Health (IYPH) 2020. The report highlights the efforts of the IPPC in promoting and implementing plant protection measures, including the development and implementation of international standards for phytosanitary measures (ISPMs), which are guidelines for countries to follow in order to prevent the spread of plant pests. The report also discusses the challenges faced by the IPPC in the face of the COVID-19 pandemic, including the need to ensure the continuity of essential plant protection activities while taking into account public health and safety measures. Overall, the report emphasizes the importance of plant protection in ensuring global food security, trade, and biodiversity conservation, and the need for continued collaboration and coordination among countries and stakeholders to achieve these goals.

Living with rodent pests: Unifying stakeholder interests to prioritise pest management in rural Madagascar

Rodent pests can have major social, economic, and environmental impacts. Their management, therefore, represents a complex socio-ecological problem involving a network of stakeholders from across different sectors, with diverging and sometimes competing interests. Failure to incorporate stakeholder interests can result in ineffective or unsustainable management programmes, with unintended negative consequences for people and nature.Participatory approaches to decision-making have been proposed as suitable strategies to tackle complex problems, yet, these processes are often considered too difficult, costly, or time-consuming to implement.To facilitate a participatory approach to rodent control in Madagascar, we identified and mapped key stakeholders and developed a multisector framework for guiding rodent management programmes based on current literature and expert recommendations. We then carried out interviews and focus groups with stakeholders and end-users to validate the final framework.The final framework unifies stakeholder interests around the dimensions of People, Resources, Knowledge and Power. Combined application of the stakeholder map and framework provides decision-makers with the tools to identify stakeholder interests;to explore areas of conflict, as well as areas of agreement;and to ensure that these are addressed within the design of control programmes. As an assessment tool, the framework can also be used to evaluate the responsiveness of programmes to the needs of different stakeholders and assess whether objectives are being reached.We recommend the application of the stakeholder map and framework to encourage and strengthen participatory approaches aimed at rodent pest control. Due to the inclusive and interdisciplinary nature of the framework, it can be applied to address numerous complex social, environmental, and economic issues across scales, sectors, and systems.Read the free Plain Language Summary for this article on the Journal blog.

Precision biological pest control of horticultural crops. (L'Accademia per il post Covid-19.)

Precision biological control of horticultural pests. Modern agriculture is developing new strategies and technologies to reduce the environmental impact and to fit new market requirements. In this scenario, a forthcoming major challenge is the reduction of insecticide and acaricide use. Herein, we propose a precision agriculture approach for managing arthropod pests in greenhouses, based on the use of artificial intelligence for monitoring and forecasting pest outbreaks, to improve timing and efficacy of biological control agent releases.

Effect of Metarhizium anisopliae on the nutritional physiology of the fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

Background: The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), was recorded for the first time in Pakistan in 2019, and now it has spread in several regions, i.e., Punjab and Sindh, affecting maize production. Farmers are using widely synthetic pesticides to control the pest. Further, the resistance development in insects and the non-target effect of chemicals on the environment and humans pose serious threats of using insecticides. The use of entomopathogenic fungi (EPF) is being considered an important tool in integrated pest management program. The main objective of this study was to check the impact of different conidial concentrations of Metarhizium anisopliae fungus on the nutritional physiology of S. frugiperda. Results: The dose-dependent effect of tested EPF was observed, and the highest concentration (1 × 109 conidia/ml) considerably affected nutritional parameters. Reduced relative consumption rate (21.7%), relative growth rate (19.5%), the efficiency of conversion of ingested food (24.2%), and approximate digestibility (16.3%) were observed in treated larvae compared to the untreated by using a higher concentration of EPF. Pupal weight was also found lower (77.9 mg and 84.2 mg, respectively), when larvae were treated by 1 × 109 and 1 × 108 conidia/ml concentrations of EPF. Further, 46.7% of larvae were found dead with this conidial concentration. Conclusions: This study suggests that the application of a higher concentration of M. anisopliae could be an effective option to control S. frugiperda. The EPF can enhance the integrated pest management programs and could be useful in reducing the environmental impact of synthetic insecticides. [ FROM AUTHOR] Copyright of Egyptian Journal of Biological Pest Control is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)

When Scent Becomes a Weapon—Plant Essential Oils as Potent Bioinsecticides

Crop protection still mostly relies on synthetic pesticides for crop pest control. However, the rationale for their continued use is shaded by the revealed adverse effects, such as relatively long environmental persistence that leads to water and soil contamination and retention of residues in food that brings high risks to human and animal health. As part of integrated pest management, biopesticides may provide crop protection, being eco-friendly and safe for humans and non-target organisms. Essential oils, complex mixtures of low-molecular-weight, highly volatile compounds, have been highlighted as major candidates for plant-derived bioinsecticides that are up to the sustainable biological standard. In this review, we screened the insecticidal activity of essential oils or their purified compounds, with focus given to their modes of action, along with the analyzed advantages and problems associated with their wider usage as plant-derived insecticides in agriculture.

Trends in Ornamental Plant Production

Other factors important for the constant expansion of the floriculture industry include implementing new strategies for plant reproduction, regulating their growth and development, adapting production technologies to fit the idea of sustainable development, and optimizing supply chain management [4,5]. The environmental management technique called ‘life cycle assessment’ (LCA) is a tool defined in ISO standards and recommended in many EU documents. The authors point out that growing C. persicum in accordance with the principles of integrated pest management and using compost to significantly limit the consumption of mineral fertilizers brings about measurable benefits for the environment and human health. The long-term development of this industry, faced with the current energy crisis, post-pandemic challenges, and threats to global geopolitical stability, is highly uncertain. [...]to continue its constant development, it is necessary to adapt cultivation methods to actual conditions and take into account the energy transformation and biological, technical, and organizational advances.

Incidence and Severity of Aphid-transmitted Viruses and Horticultural Performance of Habanero Pepper (Capsicum chinense Jacq.) Breeding Lines in Benin

Habanero (Capsicum chinense Jacq.) is widely grown and consumed in West and Central African countries, and viral diseases represent an important production challenge. Diagnosis of the viral species affecting habanero productivity in Benin is limited, and understanding this will enable more efficient host resistance breeding. During 2019 and 2020, we characterized the incidence and severity of the viral diseases infecting nine promising habanero breeding lines and one commercial hybrid check under open field conditions in Benin. The horticultural performance, including yield and yield component traits of the entries, was determined during the 2 years of the experiment. A randomized complete block design was used with three replications, each with 24 plants. Data were recorded on days to 50% flowering and 50% fruit maturity, yield and on the yield components of fruit weight (g), fruit length (cm), and fruit width (mm), as well as disease incidence and severity. In total, 35 leaf samples were collected for viral diagnosis among habanero breeding lines. We found that Pepper veinal mottle virus (PVMV;Potyvirus) was the overwhelmingly predominant virus in our trials, with an 80% incidence;however, we found frequent coinfection of PVMV with Cucumber mosaic virus (CMV, Cucumovirus), Polerovirus, and, to a lesser extent, Chili veinal mottle virus (ChiVMV;Potyvirus). The mean disease incidence across all entries was 60%. AVPP1932 and PBC 2010 had the lowest disease incidence (35% and 43%, respectively), whereas AVPP1929 had the highest (86%) disease incidence. The F1 hybrid check Afadja had the overall highest yield, with 30 t·ha-1, followed by AVPP1932, with 19 t·ha-1, both in 2019. There was a negative correlation between disease incidence and total yield (r 5 20.44;P < 0.001), supporting previous studies indicating that viral diseases are major production constraints for habanero in West Africa. This study provides insight regarding the need to improve habanero for resistance to aphid-transmitted viruses and develop integrated pest management strategies to limit losses in Benin. [ FROM AUTHOR] Copyright of HortScience is the property of American Society for Horticultural Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)

Status of Pesticide Usage on Golf Courses in Korea and Optimal Pesticide Usage Plan

Risks to human health and the environment owing to pesticide usage have arisen interest, increasing the demand for reducing pesticide consumption used on golf courses. However, standard guidelines or manuals for reducing pesticide usage on golf courses in Korea are lacking. Herein, the trends of pesticides on golf courses were investigated, and the optimal pesticide usage plan was proposed for continuous pesticide reduction. In 2019, there were 539 golf courses in Korea. With the increasing number of golf courses in 2010–2019, pesticide usage increased continuously. Fungicides accounted for more than half the pesticides used, followed by insecticides and herbicides. Except for golf courses that do not employ chemical pesticides, pesticide usage per unit area varied in the range of 0.02–65.81 kg ha−1 (average of 6.97 kg ha−1). In the US, best management practices and integrated pest management (IPM) have been stipulated and are operational in each state for pesticide management in golf courses, recognizing chemical pesticide usage to be the last approach for pest control and turf health maintenance. Considering that Korea globally ranks 10th in the number of golf facilities and courses, the establishment of IPM practices suitable for domestic conditions is essential.

Housing and Environmental Enrichment of the Domestic Ferret: A Multi-Sector Survey

Simple SummaryHow ferrets across sectors are housed and the environmental enrichment provided (e.g., toys, beds, exploration of new sights and smells) can directly impact their health and wellbeing. Through an online questionnaire reaching ferret caretakers from pet owner, laboratory, zoo, rescue, and working (e.g., pest control) sectors, we describe how ferrets are housed, the enrichment they receive, enrichment types that ferrets most enjoy and those which may be harmful or problematic. Of 754 responses, 82.4% were from pet owners. Most ferrets were housed with at least one other ferret, and the type of housing varied across sectors from single-level cages to free-ranging housing. Environmental enrichments most commonly reported were hammocks, tunnels and human interaction, with ferrets reported to most enjoy digging, tunnels, human interaction and exploration. Scent trails were also reported to be among the most enjoyable enrichments but were rarely provided, suggesting that they could be used more. Problematic enrichment included rubber items, such as Kongs®, which could be chewed and swallowed, narrow tunnels trapping ferrets, and fabrics catching claws. These items should therefore be avoided. Our results suggest that all sectors have room to improve both housing and enrichment to better ferret welfare.Ferrets (Mustela putorius furo) are kept and used in multiple sectors of society, but little is known about how they are housed and what environmental enrichment (EE) they may benefit from. We aimed to help guide caretakers about what housing and EE can be provided for ferrets. Through an online questionnaire of ferret caretakers, including pet, laboratory, zoological collection, rescue and working animal sectors internationally, we described ferret housing, opportunities for exploration, EE provision and caretaker opinions on ferrets’ preferred EE types, and problematic EE. In total, 754 valid responses from 17 countries were analysed, with most (82.4%) coming from pet owners. Most ferrets were housed socially, with housing varying across sectors from single-level cages to free-range housing in a room or outdoor enclosure;pet owners mostly used multi-level cages. The most commonly reported EE included hammocks, tunnels and tactile interaction with caretakers. Respondents reported that ferrets particularly enjoyed digging substrates, tunnels, human interaction and exploration. The most frequently reported problems were that ingestion of unsuitable chew toys and rubber items could cause internal blockages, narrow tunnels could trap ferrets, and certain fabrics that could catch claws. This suggests a need for increased awareness of the risks of these EE types and for more commercially available safety-tested ferret EE. Scent trails were relatively rarely provided but were reported to be enjoyed and harmless, so we recommend that these should be provided more commonly. Our results suggest that there is scope to improve ferret housing and EE provision to benefit ferret welfare across all sectors.

Impact of the fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), invasion on maize and the native Spodoptera litura (Fabricius) in East Java, Indonesia, and evaluation of the virulence of some indigenous entomopathogenic fungus isolates for controlling the pest

Background: The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is an invasive alien species in Indonesia. This study aimed to assess the impact of its invasion in Indonesia by measuring the damaged area caused by the pest in maize fields located across East Java and simultaneously determine whether S. frugiperda outcompetes the native Asian armyworm Spodoptera litura (Fabricius). Secondly, the virulence of 14 entomopathogenic fungus (EPF) isolates against S. frugiperda larvae was evaluated in an effort to find effective biocontrol agent candidates. Results: The damaged area caused by S. frugiperda was generally higher than that caused by S. litura during the survey period from August 2019 to December 2021. It indicated that S. frugiperda may have dominated the native armyworm and become the primary key pest of maize in Indonesia. Based on a single-concentration assay (106 conidia ml−1), the tested EPF isolates displayed varying degrees of virulence against S. frugiperda larvae, causing larval mortality of 3.5 to 71% at 10-day post-treatment, with the highest mortality rates provided by Beauveria bassiana sensu lato and Trichoderma asperellum sensu lato. At a concentration of 108 conidia ml−1, B. bassiana s.l. and T. asperellum s.l. elicited high larval mortality of 76 and 81%, respectively, at 10-day post-treatment. Nevertheless, the probit analysis based on a concentration–response assay revealed that T. asperellum s.l. had lower LC50 and LC90 values than B. bassiana s.l. Conclusions: The attack and invasion of S. frugiperda seem to be a continual threat to the maize agro-ecosystem in Indonesia. As a consequence, Indonesia should mitigate and be well-prepared for future outbreaks of S. frugiperda. Indigenous EPF isolates used in this study may act as promising biocontrol agents of S. frugiperda, especially T. asperellum s.l. This study also serves as the first report documenting the direct lethality of Trichoderma fungus on S. frugiperda larvae. [ FROM AUTHOR] Copyright of Egyptian Journal of Biological Pest Control is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)

Abstracts from the 21st Australia and New Zealand Microcirculation Society Symposium

The proceedings contain 32 papers. The topics discussed include: characterization of pericyte changes in healthy and type 2 diabetic muscles;using a PDGFR-CREERT2 transgenic mouse line to deplete pericytes in the brain;megadose vitamin C: a new therapeutic to reverse renal microcirculatory dysfunction in sepsis and COVID-19;stroke accentuates age-dependent neutrophil impairment;biological control of adipose tissue repair - implications for healing of surgical wounds;circulating CCR6+ ILC levels are altered in alemtuzumab-treated multiple sclerosis patients;cladribine alters lymphocyte trans-endothelial migration via CD49D expression in multiple sclerosis patients;extracellular vesicles and mimetic technologies for theranostics;and novel phosphorescent stain for microvesicle penetration through brain microvascular endothelium.

Prospects of the Application of Garlic Extracts and Selenium and Silicon Compounds for Plant Protection against Herbivorous Pests: A Review

Protection of plants against herbivorous pests is an important aspect that guarantees agricultural efficiency, i.e., food provision to populations. Environmental, water and foodstuff pollution by toxic pesticides, along with climate changes, highlight the necessity to achieve intensive development of ecologically safe methods of herbivory control. This review discusses modern methods of plant protection against insect pests: the biofortification of plants with selenium, treatment of plants with bulk and nano-silicon, and utilization of garlic extracts. The peculiarities of such methods of defense are described in relation to growth stimulation as well as increasing the yield and nutritional value of products. Direct defense methods, i.e., mechanical, hormonal, through secondary metabolites and/or mineral element accumulation, and indirect defense via predator attraction are discussed. Examples of herbivorous pest control during plant growth and grain/seed storage are emphasized. A comparison of sodium selenate, silicon containing fertilizer (Siliplant) and garlic extract efficiency is analyzed on Raphanus sativus var. lobo infested with the cruciferous gall midge Contarinia nasturtii, indicating the quick annihilation of pests as a result of the foliar application of garlic extract or silicon-containing fertilizer, Siliplant.

Deficit Water Irrigation in an Almond Orchard Can Reduce Pest Damage

Irrigated almond orchards in Spain are increasing in acreage, and it is pertinent to study the effect of deficit irrigation on the presence of pests, plant damage, and other arthropod communities. In an orchard examined from 2017 to 2020, arthropods and diseases were studied by visual sampling under two irrigation treatments (T1, control and T2, regulated deficit irrigation (RDI)). Univariate analysis showed no influence of irrigation on the aphid Hyalopterus amygdali (Blanchard) (Hemiptera: Aphididae) population and damage, but Tetranychus urticae Koch (Trombidiformes: Tetranychidae) damage on leaves was significantly less (50–60% reduction in damaged leaf area) in the T2 RDI treatment compared to the full irrigation T1 control in 2019 and 2020. Typhlocybinae (principal species Asymmetrasca decedens (Paoli) (Hemiptera: Cicadellidae)) population was also significantly lower under T2 RDI treatment. Chrysopidae and Phytoseiidae, important groups in the biological control of pests, were not affected by irrigation treatment. The most important diseases observed in the orchard were not, in general, affected by irrigation treatment. The multivariate principal response curves show significant differences between irrigation strategies in 2019 and 2020. In conclusion, irrigation schemes with restricted water use (such as T2 RDI) can help reduce the foliar damage of important pests and the abundance of other secondary pests in almond orchards.